Products > Reverse Transcription Series > M-MLV(reconbinant) (R1041/R1042)

M-MLV(reconbinant) (R1041/R1042)

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Contents
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Description
The  Dongsheng M-MLV Reverse Transcriptase (RT) is a genetically modified  M-MLV RT. It differs from the M-MLV RT by its structure and catalytic  properties. The enzyme possesses an RNA-dependent and DNA-dependent  polymerase activity and a ribonuclease H activity specific to RNA in  RNA-DNA hybrids. Dongsheng M-MLV Reverse Transcriptase has significantly  lower RNase H activity than Avian Myeloblastosis Virus (AMV) reverse  transcriptase.

Source
E.coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus reverse transcriptase.

Applications
Generation of first strand cDNA for use in:
-PCR, see Protocol for First-strand cDNA Synthesis;
-real-time PCR;
-second strand cDNA synthesis;
DNA labeling;
Analysis of RNA by primer extension.

Quality Control
The  absence of endodeoxyribonucleases, exodeoxyribonucleases, phosphatases  and ribonucleases confirmed by appropriate quality tests.

Functionally tested in first strand cDNA synthesis.

Store Buffer
20m M Tris-HCl (pH 7.5)
200 mM NaCl
0.1 mM EDTA
1 mM DTT
0.01%  NP-40
50%  glycerol

5xFirst-Strand Buffer
250 mM Tris-HCl (pH 8.3 at 25℃)
375 mM KCl
15 mM MgCl2
50 mM DTT